The story takes place in Metro City, circa 2000 A.D. After the death of his son, Dr. Tenma (Dr. Boynton) copes with his grief by creating a robot child that looks like his son: Astro Boy. After some time it becomes clear that Astro is not like Tobi, so his father sells him to Hamegg (Cacciatore), the cruel ringmaster of a robotic circus. At the circus, Astro is forced to fight against Z.O.G. and wins after he discovers some of his abilities. Professor Ochanomizu (Dr. Elefun) protests the abuse of robots and unsuccessfully negotiates for Astro's release. Later that night, Astro revives some robotics that Hamegg plans to sell as scrap metal. Later a robot named Sparky explodes, causing the whole circus tent to collapse. Astro leads the robots in a rescue mission. Later at a hospital, Astro is liberated from Hamegg as Professor Ochanomizu leaves with him.

Astro Boy longs to have parents, so Professor Ochanomizu has robot parents custom made. In a robotic factory, an experimental robotic named Frankenstein (Colosso) goes haywire and escapes. Frankenstein becomes the slave of a gang led by Acetylene Lamp (Big Boss), who has Frankenstein steal from a safe, a ballet, and a bank. Astro is blamed by Inspector Tawashi (Inspector Gumshoe) for the crimes, but is saved by Professor Ochanomizu. Professor Ochanomizu devises a plan to bait Frankenstein onto a ship using fake diamonds, where Astro defeats Frankenstein. The mobsters arrive, but Astro defeats them and they are soon arrested. Astro is rewarded for his heroism with his recently completed parents. Frankenstein is repaired.

When the first expedition to Mars fails because of a mutiny by the crew, Astro Boy is appointed commander of the second expedition.

Astro Boy and Professor Ochanomizu investigate the disappearances of campers and mountaineers in the ice mountains, and discover a scientist who unleashes a swarm of snails on the world.

Astro Boy is tricked by some bandits into helping them search for treasure in the Egyptian desert.

An invisible robot named Denkou (Zero) is stolen by the gangster Skunk Kusai (Fearless Fred Fink), who seeks to use him for criminal purposes. Denkou has no concept of good or bad because of his upbringing under the criminal, but is saved by Astro.

Alien resembling aliens including the doctor of Ochanomizu and Higeyajiji emigrated from the stars that looked like the earth in emergency. However, the food crisis and humankind will begin attacking the aliens with cell shrink solution.

In a faraway land, a dictator rises to power and seeks to rule the world.

Criminal Black Lux (Sharkey Dirk) becomes notorious in the Arctic.

Astro Boy leaps aboard a spaceship just before blastoff.

Higeoyaji is relaxing at home when suddenly a mysterious ship from the future crashes through his front door, the work of Dr. Time.

Cross Island is a mysterious uncharted island where a task force of robots work in great secrecy, extracting valuable stores of uranium.

That priest was dying carving a single mark on the eyes of Christ decorated in the church. Apparently it seems to be a clue to teach the identity of the pirate who broke into the church. The same pirate begins to aim for beard hair that knew their secret.

Film producer Elia Belial plans to ruin Earth using an artificial sun.

Astro runs into two of his friends and stumbles upon an interesting flower. While Astro's friends want to pick it, Astro says to leave the flower alone. He then proceeds to tell the tale of this bizarre flower.

Young Koichi (Tommy Speed) prepares to enter a world race with his supersonic car, the Silver Comet.

Saturn (Baron Von Hoodwink), an evil robot kidnaps Princess Odette (Princess Lollipop), a robot swan who can turn back into her normal self when everyone's asleep. She runs away from Hullabaloo Land as Astro comes to her aid.

A robot centipede disguised as 47 wrestling robots causes havoc on a ship and in the ocean.

Garon, a giant cosmic robot lands on Earth as a meteor, then Astro Boy and the scientists reconstruct him. During a thunderstorm, Garon revives. A corrupt scientist tells Garon to reconstruct an island in the image of his home planet, also changing the atmosphere to match that of the planet, which is toxic to life on Earth.

Astronauts report seeing fireflies in space, but they are really gaseous beings led by the evil Toxor.

At the large space station Satellite R-45, Astro Boy discovers a pair of souvenir vendors hawking stolen wares.

At a beach Astro Boy spots a mysterious bottle with a note calling for rescue from an uncharted island called Sea Serpent Isle.

A mysterious epidemic causes people to drop into comas.

A strange robot demands that Astro Boy and Professor Ochanomizu accompany him to an unknown kingdom at the bottom of the sea.

Together with Dr. Jordan who ran away from under the president who plots world conquest, Atom who got into the underground tank fights the president. But the underground tank loses control and starts pushing towards the volcano.

An evil scientist who hates civilization named Dr. Ram (Don Tay) invents a robot called Atlas to destroy a village.

Time after time, mysterious forces destroy the huge underwater dam being built by the Institute of Science.

Dr. Fooler (Dr. I.Q. Plenty) pitches to some plant owners a machine that makes troublesome robots wacky and therefore harmless. The plant owners accept, and the device affects every robot in the city including Astro Boy.

Once a year, the citizens of Earth celebrate Memory Day and honor the memories of family members who have traveled far into the universe.

A president fears for his life, as the presidents of several other countries have all been attacked by Führer ZZZ (Secret Agent 3-Z).

A monster appears in the city, and it turns out to be Dr. Dorian, who has been exiled to Mars. He turns into a monster because he cannot get cosmic X-rays on the Earth.

Dogs transplanted their brains to the robot are remodeled by Dr. Ponkotsu and challenge humanity as a hot dog corps.

Kino (Abracadabra), a robot magician is approached by a strange magician named Tenpercent, who later frames him for stealing art paintings from a museum. With Astro's help Kino must provide the evidence that he was framed.

The civilized world is shocked to hear of the sighting of a horrific monster.

Astro Boy sets out on a rocket to rescue people who have been kidnapped for use as livestock on a planet.

The religious group Pui Pui is looking for a wonder drug that will give them immortality. Dr. Tatsuji, who has succeeded in developing such a drug, is taken away by the religious group. So Astro Boy fights them to ensure the peaceful use of the drug.

On his birthday, Astro Boy receives a little sister, Uran.

The small planet Mezusa goes out of its orbit and heads straight to the Earth. To save Earth from a collision with the planet, Astro Boy heads for the planet with a convict who is a bomb professional.

Dr. Shisoku despairs of human beings that continue the ongoing destruction of nature, and makes animals attack cities in a machine that hypnotizes with ultra short waves.

Sam Caesar, a mad scientist hopes to create a new Roman Empire.

X Bomb, which is capable of turning any substance to water, drops into the Pacific Ocean. There are 50 hours left until the explosion. Astro Boy goes to recollect it, but learns that Uran, who has followed him, has been swallowed along with the bomb by a whale.

Disguising himself, as a human boy, Astro Boy goes undercover to investigate a drug trade. Some criminals have developed a drug called Yellow Horse, when injected it causes people to dance and make people want more. Professor Ochanomizu has been captured by the drug ring and injected. They try to blackmail him into helping them produce and distribute the drug in exchange for another shot. But Astro is able to pose as a Yellow Horse addict thanks to an artificial skin that makes it look like he is injecting it into himself. He tracks down the headquarters and leads the police to arrest them all. The Professor goes to rehab along with all the addicts for detoxification.

President Ditto (President Rag) is the first robot to lead a nation. His creator Deadcross kidnaps him, and Astro Boy must expose his plot.

Uran is kidnapped and made a hostage in an operation to steal Cleopatra's necklace from Astro Boy. The conspirators believes that whoever possesses the necklace can build the greatest empire in the world.

The gang of conspirators obtains the coveted necklace, and at last sets about conquering the world. Astro Boy stands in the way of their boss, Cleopatra.

The robot spaceship Lever Robot is stolen. Astro Boy is sent to retrieve it, but in the process has his energy stolen.

Astro must destroy a Cosmic Crab (Space Crab) from space.

Nuu, member of the Tenma Tribe, comes from the depths of the mountains of Mongolia. People rush to the city just to see Nuu, throwing the city into a big chaos. Moreover, a gang plots behind the scenes to capture Nuu.

Dr. Hanabusa develops an electrical transmitter for substances. No sooner does he get into the machine for testing that his rival scientist breaks the machine, which makes Dr. Hanabusa an invisible man.

Astro meets Heck Ben, a scientist's son who time travels back to the American wild west to be a cowboy.

Astro Boy must help an elephant Pura (Jimbo) to get back to his home.

An old man who comes from Planet Regel to the Earth brings a snow lion with him. Then aliens from Planet Regel start to spray cosmic snow that sucks up all energy. Astro Boy takes off for space to save the Earth, when cities have stopped functioning.

Astro joins forces with a group of space troopers to battle aliens who have invaded the Moon.

Humans can see the beauty of things and be impressed by them. In order to learn these feelings from the “heart," Astro, Uran and Lara start attending a robot school.

Astro and Dr. Ochanomizu decipher an ancient code of 200,000 years ago. Discovering that hidden hints in the 13 statues of God in Western Island might lead them to the secrets of outer space, they head for the island.

Gaston, who has stolen a blueprint of a robot from a UFO, requests the Science Ministry to build the robot exactly as shown on the blueprint, without indicating its source. The completed robot, Buron turns out to be a destructive weapon and starts to destroy everything.

Astro fights poisonous artificial bees.

Astro Boy and his pal Specs visit a carnival fun house where Specs is frightened by all the tricks played on them.

Dr. Zulu places a bomb in an artificial iceberg and tries to trigger a flood. Astro sets out to prevent the explosion.

Tick and Tack get lost in the depths of the Unzen Mountain, and decide to stay for a night in a deserted house inhabited by a vampire. The vampire, Dr. Pedan tries to turn Tick into a vampire, but Astro comes to rescue them.

A massive tidal wave breaks. Astro flies up to stop the wave, but crashes into the sea. Then an escapee robot Boggy saves the unconscious Astro.

In order to rid the earth of space pirates, Astro destroys their mother ship. The pirates, however, are holding some hostages and demand that he withdraw.

While playing in the toy section, Uran imbues some toys with artificial intelligence. Among these are some with evil hearts. Astro asks the good toys to help him stop the bad dolls from doing misdeeds.

Astro is babysitting in an ancient amusement park when a dragon flies off with a small child. Then the dinosaurs demand food for ransom.

Dr. Temp, who has completed his killing beam machine, is planning to ruin the Conference of Scientists. Discovering the plot, Astro takes on the doctor.

Rafflesia, the largest flower on earth, is found to be a plant man from outer space. This flower, which needs radioactivity to survive, is stronger than it looks, and even Astro is no match for it.

Bem (Beamo), an alien fugitive disguises himself as a human to get away from his planet.

Astro is chasing gangs who run away by rocket. Then he finds them in a shelter station being attacked by aliens. He helps them, but finds himself drifting in space with them after the station is destroyed.

The daughter of Dr. Putt is kidnapped. When he sees Uran, who is a lookalike of his daughter, he takes Astro and his fellows for the kidnappers.

It is thought that a highly advanced civilization might exist underground. To test this hypothesis, Astro and Uran set out on an underground expedition. There they get attacked by underground men.

In order to collect the hidden treasures of the Inca scattered around the world, the old man Kapack, who has telekinesis, asks Tondale for help. Tondale, however, plans to get all the gold to himself, and starts an attack on the world with a special machine that enhances mental power.

Dr. Morse succeeds in corresponding with Planet Paranoia, which is more civilized than the Earth. With the help of Astro, the doctor assembles a mysterious box following the instructions on a blueprint sent from the planet.

A boy named “Looks" is deeply attached to the robot that has brought him up. From the day it is destroyed by another robot, he begins to hate all robots. Then he forms an exclusive organization called “Black Looks," and starts to oppress robots.

Astro and his friends jump into the far future by accident. There, they see the cruel Pepper tribe treating their enemy tribes as slaves.

Dr. Kanekura kills world famous brain surgeon Dr. Ibal, making it look like an accident. A strange robot named Friday follows him around. In truth, Dr. Ibal's brain waves are controlling the robot.

Astro meets a strange creature in the wooded outskirts of Tokyo. It is a humanoid, a product of science. The humanoid named Bill does not know whether he is a creature or a machine.

What kind of dreams do robots have? Astro participates in an experiment of a dream machine created by Dr. Poron, and becomes the first robot that dreams. In his dream, he turns into a human.

Astro participates in the Olympic games for robots, where he finds a group of robots destroying other participants in a vicious act of sabotage.

Dr. Raja creates a robot bird. Then it comes flying over Japan. The bird Garuda demands Astro as a sacrifice.

Claiming to possess the ocean floor, Hall tries to build an autocratic submarine civilization. He starts to persecute the Dolphins. Astro stands up to preserve the peace on the ocean floor.

One of the latest means of traffic, the Beltway, is almost complete. However, someone tries to obstruct its construction, setting the site aflame. Astro sets off to protect the Beltway.

Dr. Tempo is waiting for a chance to become the next director of the Science Ministry. With a time gun that can send its targets to the past, he sends the bothersome Astro and Dr. Ochanomizu to the middle Ages.

Astro exchanges messages with a star nymph, and sets out on an expedition to find the pure water the nymph needs. The star nymph is in fact a princess of the Planet Keios. She is trying to turn into a cocoon with pure water on a full moon night in preparation for emergence.

The pilot of a rocket that has made an emergency landing is suffering from a strange disease. After they hear a voice from within the pilot's body, Astro and Higeoyaji are reduced to tiny figures, and get into the body. Inside, they see bacteria-sized aliens.

Dr. Ochanomizu accepts a request to repair robots in the Scambo Empire. The robot he repairs however turns out to be the president of the Empire. The doctor's life is in danger, as he now knows the secret.

A robot child has a fight with a human child, making the child cry. The humans become angry when they hear this, and a war breaks out between humans and robots.

Garon, the robot Astro sent back to space, comes back. Seeing Astro struggle hard to fend off Garon, the Earth Robot Army stands together to attack Garon.

In order to save patients suffering from space disease on Mars, a serum is sent to Mars from Earth. All the transport ships carrying the serum, however, get destroyed on their way. Astro, together with the three robot knights, goes into space to protect the last of the serum.

Cobalt (Jetto), a defective prototype of Astro built by Dr. Tenma, is released from storage and becomes Astro's older brother.

The construction of a dam near the Bering Strait is hampered by difficulties. Dr. Zeman's research is required to solve the problem. Astro goes to ask for help from the doctor who lives a secluded life in the depth of the Alps because of his hatred for civilization. Astro, however, finds the doctor being attacked by another robot that is after the research material.

A missile base is completely controlled by a computer. Now, the computer is out of control, setting the stage for nuclear attack. In fact, this is a means of personal revenge by Dr. Yakoref, who lost the competition for the design of the base. Astro starts to disassemble the computer.

Dr. Akuta, who is after the gold ingot on Mars, controls a robot called Future. The robot, however, becomes humble after hearing out Astro. Then Astro and Future pursue the doctor, who has run away with the gold ingot.

Planet Hyper is the most civilized planet in the galactic system. It chooses a pair of a human and a robot each from both the Earth and Planet War to have them fight against each other, and will destroy the loser's planet. Astro and Ponkotsu Tetsu, the safebreaker, are chosen to represent the Earth.

A big robot is excavated from the construction site of a tunnel. It turns out to be a time capsule that was created to pass down information on a super ancient civilization of a million years ago to posterity.

Colon-bu-bu, the explorer of the underground state of Chombo, heads for the ground hoping to see a new world. Then he meets Cobalt and takes him underground as prisoner.

On Christmas night, Dr. Ochanomizu opens the will of his scientist friend who died 10 years before. It says that he has left an invention in Bururu Mountain. Astro and his friends happen to be on the mountain and encounter a boy called Akio who lives alone there.

The visiting Prince Dorian of the Camellia Kingdom disappears. Astro goes searching for him using the latest 4D detection device, and finds the prince wandering in another world. That world is inhabited by people who have been spirited away from various time periods.

Uran is going to be in a movie as Tarzan. When she goes on location to the jungle, however, a mysterious woman takes her away. The woman is planning to make Uran, who is dressed as a boy, the prince of the demon's place.

Humans are drawn toward a flight of stairs that suddenly appear one day, and they start to walk towards a spaceship hidden among the clouds. When there are no humans left on earth, the robots start to talk about taking over the planet.

Astro is shot by a gun that ruins artificial intelligence. He loses all his memory, and is shot away into space.

Hamegg sees a UFO in the woods that seems to have made an emergency landing, and finds an alien child. He notices the child's super strength, and plans to win the child's fame as a wrestler.

Astro is involved in an explosion during the construction of a dam. The impact sends him into a parallel world where Tobio, the son of Dr. Tenma, never died in an accident. In other words, it is a world where Astro has not been born.

Three scientists gain supernatural power on the surface of the moon. Then Dr. Arden, who has a grudge against Dr. Ochanomizu, stages an attack against him. In order to protect Dr. Ochanomizu, Astro fights fiercely against the scientists who have supernatural power.

Astro finds a big egg in a cave. He asks Dr. Ochanomizu to identify the egg, but they are unsuccessful. So Astro goes back to the island, where he encounters a strange bird that lives there.

Astro is investigating a super thermal bomb that has fallen on earth. Then he heads for Mercury to find its launching site. On his way, however, Astro loses his sight in an accident, and gets involved in a power struggle with aliens on Mercury.

A country called Back suddenly withdraws from the World Peace Union, and announces to its people a ban on invention and laughter. Astro and Dr. Ochanomizu visit the country to find out what is behind this dictatorial policy.

A robot, Pluto is built to kill the 7 strongest robots in the world, therefore making him the world's most powerful robot.

Pluto succeeds in disposing of Montblanc, North #2, Brando, Gesicht, Hercules, and Photar with ease, leaving Astro as his sole remaining target. After meeting Astro, he begins to question his actions.

Planet M, shaped like a convex lens, is approaching the Earth. It reflects solar light toward the Earth, turning its surface into hell. Humans, however, cannot cooperate in tackling this global issue because they are all motivated by their own greed.

Astro and Dr. Ochanomizu wander into a space graveyard that lies outside every sphere of gravitation, drifting in space. Then an earthling named Bemski, who hates the Earth, rescues them.

Higeoyaji's pet dog Pero has a diamond that it took back from space. Dr. Fooler puts Pero's memory into the computer of Captain Dog, the robot dog, to make it find diamonds. Astro follows the robot dog, which is heading for Mars.

Astro Boy and a group of passengers from a doomed rocketliner are stranded on an asteroid with strange characteristics and a mysterious monster.